HINT1 Recombinant Monoclonal Antibody, Clone: [17E12], Unconjugated, Rabbit
Catalog Number:
CSB-RA299916A0HU
- Images (5)
| Article Name: | HINT1 Recombinant Monoclonal Antibody, Clone: [17E12], Unconjugated, Rabbit |
| Biozol Catalog Number: | CSB-RA299916A0HU |
| Supplier Catalog Number: | CSB-RA299916A0HU |
| Alternative Catalog Number: | CSB-RA299916A0HU-100UL, CSB-RA299916A0HU-50UL |
| Manufacturer: | Cusabio |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, FC, IF, IHC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Conjugation: | Unconjugated |
| Alternative Names: | Adenosine 5 monophosphoramidase antibody, Adenosine 5-monophosphoramidase antibody, HINT 1 antibody, HINT1 antibody, HINT1_HUMAN antibody, Histidine triad nucleotide binding protein 1 antibody, Histidine triad nucleotide-binding protein 1 antibody, PKCI 1 antibody, PKCI-1 antibody, PKCI1 antibody, PRKCNH1 antibody, Protein kinase C inhibitor 1 antibody, Protein kinase C interacting protein 1 antibody, Protein kinase C-interacting protein 1 antibody |
| Clonality: | Monoclonal |
| Clone Designation: | [17E12] |
| UniProt: | P49773 |
| Buffer: | Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| Purity: | Affinity-chromatography |
| Form: | Liquid |
| Target: | HINT1 |
| Antibody Type: | Recombinant Antibody |
| Application Dilute: | Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200 |
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Overlay Peak curve showing 786-O cells stained with CSB-RA299916A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4°C.Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
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Immunofluorescence staining of HepG2 cell with CSB-RA299916A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
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IHC image of CSB-RA299916A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
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IHC image of CSB-RA299916A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. |
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Western Blot Positive WB detected in: Hela whole cell lysate(30µg), HEK293T whole cell lysate(30µg), Jurkat whole cell lysate(30µg), Raji whole cell lysate(30µg), K562 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg), Mouse kidney tissue lysate(30µg), Rat kidney tissue lysate(30µg) All lanes: HINT1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 14 kDa Observed band size: 14 kDa Exposure time:2min |
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