Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free, Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reinheit:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse or human heavy or light chain isotypes.
Application Notes: Anti-Mouse IgG2b peroxidase conjugated antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency
Characterization of antibody responses induced by DNA vaccines expressing particulate and non-particulate core antigens. (a-f) Mice were immunized intradermally with 2 µg particle-coated plasmids with the gene gun (see Supplemental protocols). At d21 mice were boosted with the same vectors. The specific serum Ab responses and isotype profiles (IgG, IgG1, IgG2a) were determined 12 days post boost immunization by end-point dilution ELISA using bacterial rHBV-C149 particles as detection antigen and IgG1/IgG2a ratios were calculated. (a, b) B6 mice (n = 3/4) were immunized with pCI/stC149tat or pCI/stC149 vectors. (c, d) B6 and TLR7-/- mice (n = 3/5) were immunized with pCI/stC149tat. (e, f) B6 mice (n = 5/5) were immunized with pCI/stCAAA or pCI/stCAAAY132A plasmid DNA. Mean specific antibody titers in sera (a, c, e) and the calculated IgG1/IgG2a ratios SD (b, d, f) of representative experiments (out of two experiments performed) are shown. Where indicated, the statistical significance of differences in IgG, IgG1 and IgG2b antibody titers was determined by the unpaired Students t-test. P values of < 0.05 (*) and < 0.005 (**) were considered statistically significant.
Characterization of antibody responses induced by DNA vaccines expressing particulate and non-particulate core antigens. (a-f) Mice were immunized intradermally with 2 µg particle-coated plasmids with the gene gun (see Supplemental protocols). At d21 mice were boosted with the same vectors. The specific serum Ab responses and isotype profiles (IgG, IgG1, IgG2a) were determined 12 days post boost immunization by end-point dilution ELISA using bacterial rHBV-C149 particles as detection antigen and IgG1/IgG2a ratios were calculated. (a, b) B6 mice (n = 3/4) were immunized with pCI/stC149tat or pCI/stC149 vectors. (c, d) B6 and TLR7-/- mice (n = 3/5) were immunized with pCI/stC149tat. (e, f) B6 mice (n = 5/5) were immunized with pCI/stCAAA or pCI/stCAAAY132A plasmid DNA. Mean specific antibody titers in sera (a, c, e) and the calculated IgG1/IgG2a ratios SD (b, d, f) of representative experiments (out of two experiments performed) are shown. Where indicated, the statistical significance of differences in IgG, IgG1 and IgG2b antibody titers was determined by the unpaired Students t-test. P values of < 0.05 (*) and < 0.005 (**) were considered statistically significant.
Characterization of antibody responses induced by DNA vaccines expressing particulate and non-particulate core antigens. (a-f) Mice were immunized intradermally with 2 µg particle-coated plasmids with the gene gun (see Supplemental protocols). At d21 mice were boosted with the same vectors. The specific serum Ab responses and isotype profiles (IgG, IgG1, IgG2a) were determined 12 days post boost immunization by end-point dilution ELISA using bacterial rHBV-C149 particles as detection antigen and IgG1/IgG2a ratios were calculated. (a, b) B6 mice (n = 3/4) were immunized with pCI/stC149tat or pCI/stC149 vectors. (c, d) B6 and TLR7-/- mice (n = 3/5) were immunized with pCI/stC149tat. (e, f) B6 mice (n = 5/5) were immunized with pCI/stCAAA or pCI/stCAAAY132A plasmid DNA. Mean specific antibody titers in sera (a, c, e) and the calculated IgG1/IgG2a ratios SD (b, d, f) of representative experiments (out of two experiments performed) are shown. Where indicated, the statistical significance of differences in IgG, IgG1 and IgG2b antibody titers was determined by the unpaired Students t-test. P values of < 0.05 (*) and < 0.005 (**) were considered statistically significant.
ELISA Results of Rabbit Anti-Mouse IgG2b Antibody Peroxidase Conjugation tested against purified Mouse IgG2b HRP. Each well was coated in duplicate with 1.0 µg of Mouse IgG2b (p/n orb343778). The working dilution is 1:82000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed usin
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