The immunogen is a synthetic peptide directed towards the N terminal region of human BAX
Alternative Synonym:
BCL2L4
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
Researcher: Rosa Carotenuto, Department of Structural and Functional Biology, University of Naples Federico II Application: Western blotting Species + Tissue/Cell type: xenopus laevis embryos How many ugs of tissue/cell lysate run on the gel: 1. 50 ug xenopus laevis embryos lysate (lysate buffer) 2. 50 ug xenopus laevis embryos lysate (HB buffer) Primary antibody dilution: 1:400 Secondary antibody: Goat Anti-Rabbit AP Secondary antibody dilution: 1:20000
BAX antibody - N-terminal region (ARP58870_P050) in Human HepG2 using Western Blot
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