Tyrosine Hydroxylase Recombinant Rabbit Monoclonal Antibody, Clone: [8B6], Unconjugated
Artikelnummer:
BYT-ORB1499388
- Bilder (7)
| Artikelname: | Tyrosine Hydroxylase Recombinant Rabbit Monoclonal Antibody, Clone: [8B6], Unconjugated |
| Artikelnummer: | BYT-ORB1499388 |
| Hersteller Artikelnummer: | orb1499388 |
| Alternativnummer: | BYT-ORB1499388-100,BYT-ORB1499388-50 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | A synthesized peptide derived from human Tyrosine hydroxylase (500-528aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | DYT14, DYT5b, TYH, The, TY3H_HUMAN, TH, Tyrosine 3-hydroxylase (TH), 1.14.16.2, TY3H_MOUSE, TY3H_RAT, |
| Tyrosine Hydroxylase Recombinant Rabbit Monoclonal Antibody |
| Klonalität: | Recombinant |
| Konzentration: | 1mg/ml |
| Klon-Bezeichnung: | [8B6] |
| Molekulargewicht: | 60 kDa |
| UniProt: | P07101 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | TH |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1:50-100 |
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ICC staining of Tyrosine Hydroxylase in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (orb1499388, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue). |
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ICC staining of Tyrosine Hydroxylase in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (orb1499388, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue). |
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ICC staining of Tyrosine Hydroxylase in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (orb1499388, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1000 dilution. The nuclear counter stain is DAPI (blue). |
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Paraformaldehyde-fixed, paraffin embedded (human adrenal gland), Antigen retrieval by boiling in EDTA buffer buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Tyrosine Hydroxylase) Monoclonal Antibody, Unconjugated (orb1499388) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in EDTA buffer buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Tyrosine Hydroxylase) Monoclonal Antibody, Unconjugated (orb1499388) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland), Antigen retrieval by boiling in EDTA buffer buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (Tyrosine Hydroxylase) Monoclonal Antibody, Unconjugated (orb1499388) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Mouse Adrenal gland tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Rat Adrenal gland tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Primary: Anti-Tyrosine Hydroxylase (orb1499388) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60 kDa, Observed band size: 60 kDa. |
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