ERG Recombinant Rabbit Monoclonal Antibody, Clone: [2F3], Unconjugated
Artikelnummer:
BYT-ORB1499398
- Bilder (7)
| Artikelname: | ERG Recombinant Rabbit Monoclonal Antibody, Clone: [2F3], Unconjugated |
| Artikelnummer: | BYT-ORB1499398 |
| Hersteller Artikelnummer: | orb1499398 |
| Alternativnummer: | BYT-ORB1499398-100,BYT-ORB1499398-25,BYT-ORB1499398-50 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | A synthesized peptide derived from human ERG (400-479aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | LMPHM14, erg-3, p55, D030036I24Rik, ERG_HUMAN, ERG, Transforming protein ERG, ERG_MOUSE, |
| ERG Recombinant Rabbit Monoclonal Antibody |
| Klonalität: | Recombinant |
| Konzentration: | 1mg/ml |
| Klon-Bezeichnung: | [2F3] |
| Molekulargewicht: | 55 kDa |
| UniProt: | P11308 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | ERG |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:50-200, IF=1:100-500, Flow-Cyt=1:50-100 |
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25 ug total protein per lane of various lysates probed with ERG monoclonal antibody, unconjugated (orb1499398) at 1:500 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. |
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Cell line: THP-1, Fixation: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:100, Primary Ab incubation condition: 4C overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1499398. |
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Cell line: THP-1, Fixation: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary Ab Dilution: 1:100, Secondary Ab: Goat Anti-Rabbit IgG, Unlabelled control: The cell without incubation with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line). Comment: Line red is the positive signal for orb1499398. |
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Tissue: Human kidney, Section type: Formalin-fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499398. |
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Tissue: Human prostatic cancer, Section type: Formalin-fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499398. |
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Tissue: Mouse cerebrum, Section type: Formalin-fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499398. |
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Tissue: Rat kidney, Section type: Formalin-fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1499398. |
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