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Rabbit CTCF Recombinant Monoclonal Antibody, Clone: [BLR041F], Unconjugated

Rabbit CTCF Recombinant Monoclonal Antibody, Clone: [BLR041F], Unconjugated

Artikelnummer: BYT-ORB1519896

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Artikelname:	Rabbit CTCF Recombinant Monoclonal Antibody, Clone: [BLR041F], Unconjugated
Artikelnummer:	BYT-ORB1519896
Hersteller Artikelnummer:	orb1519896
Alternativnummer:	BYT-ORB1519896-100,BYT-ORB1519896-20
Hersteller:	Biorbyt
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	FC, ICC, IHC, IP, WB
Spezies Reaktivität:	Human, Mouse
Immunogen:	Between 677 and C-terminus
Konjugation:	Unconjugated
Alternative Synonym:	11 zinc finger transcriptional repressor, 11-zinc finger protein, CCCTC-binding factor, CCCTC-binding factor (zinc finger protein), CTCFL paralog, MRD21, transcriptional repressor CTCF

CTCF Antibody

Klonalität:	Recombinant
Konzentration:	100 µg/ml
Klon-Bezeichnung:	[BLR041F]
NCBI:	006556
UniProt:	P49711
Puffer:	Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Formulierung:	Liquid
Target-Kategorie:	CTCF

Application Verdünnung:	WB - 1:1000, IP - 20 ul/1mg lysate, IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections., ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Anwendungsbeschreibung:	Application Notes: Format: Whole IgG

	Detection of mouse CTCF by immunocytochemistry. Sample: FFPE section of CT26 cells. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (orb1519896). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human CTCF by immunocytochemistry. Sample: FFPE section of MCF-7 cells. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (orb1519896). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human CTCF by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (orb1519896). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of mouse CTCF by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (orb1519896). Secondary: HRP-conjugated goat anti-rabbit IgG.
	Detection of human CTCF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction, 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CTCF recombinant monoclonal antibody [BL-395-3B3] (orb1519896) used for IP at 20 µl/mg lysate.
	Detection of mouse CTCF by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BL-395-3B3] (orb1519896) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
	Detection of human CTCF by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, Jurkat (10 µg) , Hep-G2, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BL-395-3B3] (orb1519896) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

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