ALDH1A1 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB155630
Artikelname: ALDH1A1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB155630
Hersteller Artikelnummer: orb155630
Alternativnummer: BYT-ORB155630-50,BYT-ORB155630-100,BYT-ORB155630-200
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse
Immunogen: KLH conjugated synthetic peptide derived from human ALDH1 (201-300/501aa)
Konjugation: Unconjugated
Alternative Synonym: ALDC, ALDH-E1, ALDH1, ALDH11, HEL-9, HEL-S-53e, HEL12, PUMB1, RALDH1, ALHDII, Ahd-2, Ahd2, Aldh1a2, E1, Aldh2, AL1A1_HUMAN, ALDH1A1, 3-deoxyglucosone dehydrogenase, Aldehyde dehydrogenase family 1 member A1, Aldehyde dehydrogenase, cytosolic, Retinal dehydrogenase 1 (RALDH 1 | RalDH1), 1.2.1.19, 1.2.1.28, 1.2.1.3, 1.2.1.36, AL1A1_MOUSE, AL1A1_RAT, Aldh, AL1A1_SHEEP,
ALDH1A1 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 54 kDa
UniProt: P00352
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: ALDH1A1
Application Verdünnung: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of human lung cancer tissue using ALDH1A1 antibody.
Western blot analysis of A549 Human Cell Lysate,HepG2 Human Cell Lysate using ALDH1A1 antibody.
Paraformaldehyde-fixed, paraffin embedded (human liver cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ALDH1) Polyclonal Antibody, Unconjugated (orb155630) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (ALDH1) Polyclonal Antibody, Unconjugated (orb155630) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: A549 Cell (Human) Lysate at 40 ug, HepG2 Cell (Human) Lysate at 40 ug, Primary: Anti-ALDH1 (orb155630) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54kD, Observed band size: 54kD.
Sample: Liver (mouse) Lysate at 40 ug, Primary: Anti-ALDH1 (orb155630) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.
Sample: Placenta (Mouse) Lysate at 40 ug, Primary: Anti-ALDH1 (orb155630) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.