ASCL2 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB155740
- Bilder (7)
| Artikelname: | ASCL2 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB155740 |
| Hersteller Artikelnummer: | orb155740 |
| Alternativnummer: | BYT-ORB155740-50,BYT-ORB155740-100,BYT-ORB155740-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, ICC, IF, IHC-Fr, IHC-P |
| Spezies Reaktivität: | Human, Mouse |
| Immunogen: | KLH conjugated synthetic peptide derived from Human ASCL2 (51-120/193aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | ASH2, HASH2, MASH2, bHLHa45, 2410083I15Rik, ASCL2_HUMAN, ASCL2, ASH-2, Class A basic helix-loop-helix protein 45 (bHLHa45), ASCL2_MOUSE, mASH-2, ASCL2_RAT, |
| ASCL2 Rabbit Polyclonal Antibody |
| Application Verdünnung: | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test |
| Anwendungsbeschreibung: | Application Notes: IHC-P/ IHC-F (Human, Mouse): 1:100-500 IF (Human, Mouse): 1:100-500 FC (Human): 2ug/Test ICC/IF (Human): 1:100-500 |
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Immunohistochemical analysis of mouse placenta tissue using ASCL2 antibody. |
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Blank control: K562. Primary Antibody (green line): Rabbit Anti-ASCL2 antibody (orb155740), Dilution: 2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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MCF7 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (ASCL2) polyclonal Antibody, Unconjugated (orb155740) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Tissue/Cell: human placenta tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-ASCL2 Polyclonal Antibody, Unconjugated (orb155740) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: mouse lung tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-ASCL2 Polyclonal Antibody, Unconjugated (orb155740) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: mouse placenta tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-ASCL2 Polyclonal Antibody, Unconjugated (orb155740) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Immunohistochemical staining of mouse placenta tissue using ASCL2 antibody. |
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