Bcl-2 Recombinant Rabbit Monoclonal Antibody, Clone: [B9E6], Unconjugated

Artikelnummer: BYT-ORB2563019
Artikelname: Bcl-2 Recombinant Rabbit Monoclonal Antibody, Clone: [B9E6], Unconjugated
Artikelnummer: BYT-ORB2563019
Hersteller Artikelnummer: orb2563019
Alternativnummer: BYT-ORB2563019-100,BYT-ORB2563019-25,BYT-ORB2563019-50
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse
Immunogen: A synthesized peptide derived from human Bcl 2 (1-239/239aa)
Konjugation: Unconjugated
Alternative Synonym: Bcl-2, PPP1R50, BCL2_HUMAN, BCL2,
Bcl-2 Recombinant Rabbit Monoclonal Antibody
Klonalität: Recombinant
Konzentration: 1mg/ml
Klon-Bezeichnung: [B9E6]
Molekulargewicht: 26 kDa
UniProt: P10415
Puffer: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: BCL2
Application Verdünnung: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, WB=1:500-2000
25 ug total protein per lane of various lysates probed with Bcl-2 monoclonal antibody, unconjugated (orb2563019) at 1:2000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Lymph, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.