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25 ug total protein per lane of various lysates probed with Bcl-2 monoclonal antibody, unconjugated (orb2563019) at 1:2000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. |
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Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Human Lymph, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Mouse Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |