MYH1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB2563457
- Bilder (8)
| Artikelname: | MYH1 Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB2563457 |
| Hersteller Artikelnummer: | orb2563457 |
| Alternativnummer: | BYT-ORB2563457-100,BYT-ORB2563457-200,BYT-ORB2563457-50 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | Recombinant human MYH1 protein (1-350/1939aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | HEL71, MYHSA1, MYHa, MyHC-2X/D, MyHC-2x, A530084A17Rik, IId, IId/x, MHC-2X/D, MHC2X/D, MYHC-IIX, MdMs, MyHC-IId/x, MyHC-IIx/d, Myhs-f, Myhs-f2, Myhsf2, MYHC, MYH1_BOVIN, MYH1, Myosin heavy chain 1, Myosin heavy chain 2x (MyHC-2x), Myosin heavy chain, skeletal muscle, adult 1, MYH1_HUMAN, Myosin heavy chain IIx/d (MyHC-IIx/d), MYH1_MOUSE, MYH1_PIG, |
| MYH1 Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 223 kDa |
| UniProt: | P12882 |
| Puffer: | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | MYH1 |
| Application Verdünnung: | IHC-P=1:100-500, WB=1:500-2000, IHC-F=1:100-500, IF=1:100-500 |
|
|
Negative Sample: Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Paraformaldehyde-fixed, paraffin embedded (human brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MYH1) Polyclonal Antibody, Unconjugated (orb2563457) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Negative Sample: Paraformaldehyde-fixed, paraffin embedded (Mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Paraformaldehyde-fixed, paraffin embedded (Mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MYH1) Polyclonal Antibody, Unconjugated (orb2563457) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Negative Sample: Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MYH1) Polyclonal Antibody, Unconjugated (orb2563457) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MYH1) Polyclonal Antibody, Unconjugated (orb2563457) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (Mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MYH1) Polyclonal Antibody, Unconjugated (orb2563457) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (mouse tongue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MYH1) Polyclonal Antibody, Unconjugated (orb2563457) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Paraformaldehyde-fixed, paraffin embedded (rat tongue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (MYH1) Polyclonal Antibody, Unconjugated (orb2563457) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
|
|
Sample: Lane 1: Recombinant human MYH1 protein, N-His (orb1974324), Primary: Anti-MYH1 (orb2563457) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: kDa, Observed band size: 44 kDa. |
Produktgarantie und fachkundiger Support
