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Paraformaldehyde-fixed, paraffin embedded (Human abdominal skin), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TROP2) Monoclonal Antibody, Unconjugated (orb2563481) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human breast), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TROP2) Monoclonal Antibody, Unconjugated (orb2563481) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TROP2) Monoclonal Antibody, Unconjugated (orb2563481) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human Cervical squamous epithelial lesions), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TROP2) Monoclonal Antibody, Unconjugated (orb2563481) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human esophagus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TROP2) Monoclonal Antibody, Unconjugated (orb2563481) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human Prostate), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TROP2) Monoclonal Antibody, Unconjugated (orb2563481) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (TROP2) Monoclonal Antibody, Unconjugated (orb2563481) at 1:200 overnight at 4C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. |
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Sample: Lane 1: Recombinant human TROP2 protein, N-His (HEK293), Primary: Anti-TROP2 (orb2563481) at 1/1000 dilution, Secondary: Alexa Fluor 790 AffiniPure Goat Anti-Mouse IgG, light chain specific, Predicted band size: 33 kDa, Observed band size: 45 kDa. |