PYGM Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer:
BYT-ORB6826
- Bilder (8)
| Artikelname: | PYGM Rabbit Polyclonal Antibody, Unconjugated |
| Artikelnummer: | BYT-ORB6826 |
| Hersteller Artikelnummer: | orb6826 |
| Alternativnummer: | BYT-ORB6826-50,BYT-ORB6826-100,BYT-ORB6826-200 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human PYGM (401-500/842aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | GSD5, PG, Muscpho, PYGM_HUMAN, PYGM, Myophosphorylase, 2.4.1.1, PYGM_MOUSE, PYGM_RAT, |
| PYGM Rabbit Polyclonal Antibody |
| Klonalität: | Polyclonal |
| Konzentration: | 1mg/ml |
| Molekulargewicht: | 97 kDa |
| UniProt: | P11217 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | PYGM |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PYGM) Polyclonal Antibody, Unconjugated (orb6826) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat bladder tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PYGM) Polyclonal Antibody, Unconjugated (orb6826) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat heart tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PYGM) Polyclonal Antibody, Unconjugated (orb6826) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PYGM) Polyclonal Antibody, Unconjugated (orb6826) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Rat tongue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PYGM) Polyclonal Antibody, Unconjugated (orb6826) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Mouse Muscle tissue lysates, Lane 2: Rat Muscle tissue lysates, Primary: Anti-PYGM (orb6826) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 97 kD, Observed band size: 97 kD. |
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IHC-P analysis of rat bladder tissue using PYGM Polyclonal Antibody at 1:400 dilution. |
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IHC-P analysis of mouse skeletal muscle using PYGM Polyclonal Antibody at 1:200 dilution. |
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