SLC15A1 Rabbit Polyclonal Antibody, Unconjugated

Artikelnummer: BYT-ORB704521
Artikelname: SLC15A1 Rabbit Polyclonal Antibody, Unconjugated
Artikelnummer: BYT-ORB704521
Hersteller Artikelnummer: orb704521
Alternativnummer: BYT-ORB704521-50,BYT-ORB704521-100
Hersteller: Biorbyt
Wirt: Rabbit
Kategorie: Antikörper
Applikation: IF, IHC-Fr, IHC-P, WB
Spezies Reaktivität: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human SLC15A1
Konjugation: Unconjugated
Alternative Synonym: HPECT1, HPEPT1, PEPT1, S15A1_HUMAN, SLC15A1, Intestinal H(+)/peptide cotransporter, Oligopeptide transporter, small intestine isoform, Peptide transporter 1,
SLC15A1 Rabbit Polyclonal Antibody
Klonalität: Polyclonal
Konzentration: 1mg/ml
Molekulargewicht: 79 kDa
UniProt: P46059
Puffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Formulierung: Liquid
Target-Kategorie: SLC15A1
Application Verdünnung: WB=1:500-1000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SLC15A1) Polyclonal Antibody, Unconjugated (orb704521) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SLC15A1) Polyclonal Antibody, Unconjugated (orb704521) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SLC15A1) Polyclonal Antibody, Unconjugated (orb704521) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SLC15A1) Polyclonal Antibody, Unconjugated (orb704521) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SLC15A1) Polyclonal Antibody, Unconjugated (orb704521) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat small intestine), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (SLC15A1) Polyclonal Antibody, Unconjugated (orb704521) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Liver (Mouse) Lysate at 40 ug, Lane 2: Liver (Rat) Lysate at 40 ug, Lane 3: Panc-1 (Human) Cell Lysate at 30 ug, Primary: Anti-SLC15A1 (orb704521) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 80 kD, Observed band size: 83 kD.
IHC-P analysis of rat kidney using SLC15A1 Polyclonal Antibody at 1:200 dilution.