Histone H1.2 Recombinant Rabbit Monoclonal Antibody, Clone: [9C9], Unconjugated
Artikelnummer:
BYT-ORB783428
- Bilder (8)
| Artikelname: | Histone H1.2 Recombinant Rabbit Monoclonal Antibody, Clone: [9C9], Unconjugated |
| Artikelnummer: | BYT-ORB783428 |
| Hersteller Artikelnummer: | orb783428 |
| Alternativnummer: | BYT-ORB783428-50,BYT-ORB783428-100 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Histone H1.2 |
| Konjugation: | Unconjugated |
| Alternative Synonym: | H1.2, H1C, H1F2, H1s-1, HIST1H1C, H12_HUMAN, H1-2, Histone H1c, Histone H1d, Histone H1s-1, |
| Histone H1.2 Recombinant Rabbit Monoclonal Antibody |
| Klonalität: | Recombinant |
| Konzentration: | 1mg/ml |
| Klon-Bezeichnung: | [9C9] |
| Molekulargewicht: | 21 kDa |
| UniProt: | P16403 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | H1-2 |
| Application Verdünnung: | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200 |
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Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H1.2) Monoclonal Antibody, Unconjugated (orb783428) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human lung carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H1.2) Monoclonal Antibody, Unconjugated (orb783428) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H1.2) Monoclonal Antibody, Unconjugated (orb783428) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Histone H1.2) Monoclonal Antibody, Unconjugated (orb783428) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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PC-3M cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Histone H1.2) monoclonal Antibody, Unconjugated (orb783428) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Sample: Lane 1: Hela cell lysate, Lane 2: 293 cell lysate, Lane 3: MCF-7 cell lysate, Primary: Anti-Histone H1.2 (orb783428) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 21 kD, Observed band size: 25 kD. |
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Sample: Lane 1: rat liver tissue lysate, Lane 2: mouse lung tissue lysate, Primary: Anti-Histone H1.2 (orb783428) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 21 kD, Observed band size: 25 kD. |
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SK-Br-3 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (Histone H1.2) monoclonal Antibody, Unconjugated (orb783428) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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