EGFR Recombinant Rabbit Monoclonal Antibody, Clone: [3A8], Unconjugated
Artikelnummer:
BYT-ORB783429
- Bilder (8)
| Artikelname: | EGFR Recombinant Rabbit Monoclonal Antibody, Clone: [3A8], Unconjugated |
| Artikelnummer: | BYT-ORB783429 |
| Hersteller Artikelnummer: | orb783429 |
| Alternativnummer: | BYT-ORB783429-50,BYT-ORB783429-100 |
| Hersteller: | Biorbyt |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | FC, ICC, IF, IHC-Fr, IHC-P, WB |
| Spezies Reaktivität: | Human, Mouse, Rat |
| Immunogen: | A synthesized peptide derived from human EGFR (1080-1110aa) |
| Konjugation: | Unconjugated |
| Alternative Synonym: | ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA, 9030024J15Rik, Errb1, Wa5, wa-2, wa2, ErbB-1, EGFR_HUMAN, EGFR, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, 2.7.10.1, EGFR_MOUSE, |
| EGFR Recombinant Rabbit Monoclonal Antibody |
| Klonalität: | Recombinant |
| Konzentration: | 1mg/ml |
| Klon-Bezeichnung: | [3A8] |
| Molekulargewicht: | 130 kDa |
| UniProt: | P00533 |
| Puffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Formulierung: | Liquid |
| Target-Kategorie: | EGFR |
| Application Verdünnung: | WB=1:2000-20000, IHC-P=1:200-500, IHC-F=1:200-500, ICC/IF=1:100-500, IF=1:200-500, Flow-Cyt=1µg/Test |
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A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (EGFR (3A8)) Monoclonal Antibody, Unconjugated (orb783429) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (EGFR (3A8)) Monoclonal Antibody, Unconjugated (orb783429) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (EGFR (3A8)) Monoclonal Antibody, Unconjugated (orb783429) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (human gastric), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (EGFR) Monoclonal Antibody, Unconjugated (orb783429) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (EGFR) Monoclonal Antibody, Unconjugated (orb783429) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Human A549 cell lysates, Lane 2: Human U251 cell lysates, Lane 3: Human HeLa cell lysates, Primary: Anti-EGFR (orb783429) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 130 kDa, Observed band size: 180 kDa. |
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IHC-P analysis of human gastric using EGFR Monoclonal Antibody at 1:200 dilution. |
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IHC-P analysis of mouse kidney using EGFR Monoclonal Antibody at 1:200 dilution. |
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