Activating Fragment 1, CAP20, CDK-interacting protein 1, CDKI, CDKN1, CDKN1A, CIP1, Cyclin-dependent kinase inhibitor 1A (p21, Cip1), DNA Synthesis Inhibitor, MDA6, Melanoma Differentiation Associated Protein 6, p21Cip1/Waf1, PIC1, SDI1, SLC12A9, Wild type p53 activated fragment 1 (WAF1)
This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdks and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C follo
Western Blot Analysis of human HeLa
Formalin-fixed, paraffin-embedded human Lung SqCC stained with p21 Mouse Monoclonal Antibody (CIP1/823).
Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p21 Mouse Monoclonal Antibody (CIP1/823).
Western Blot Analysis of human HeLa cell lysate using p21 Mouse Monoclonal Antibody (CIP1/823).
Immunofluorescence Analysis of HeLa cells labeling p21 with p21 Mouse Monoclonal Antibody (CIP1/823) followed by Goat anti-Mouse IgG-CF555 (Red). Membrane stained with Phalloidin 488 (Green)
SDS-PAGE Analysis of Purified p21 Mouse Monoclonal Antibody (CIP1/823).Confirmation of Purity and Integrity of Antibody.
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