This MAb is specific to c-erbB-2/HER-2 and shows minimal cross-reaction with other members of the family. C-erbB-2/HER-2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
ELISA (For coating, order antibody without BSA),Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Optimal dilution for a specific application should be determined.
SDS-PAGE Analysis of Purified HER-2 Mouse Monoclonal Antibody (HRB2/776). Conf
Flow Cytometric Analysis of human trypsinized MCF-7 cells using HER-2 Mouse Monoclonal Antibody (HRB2/776) followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype control (Red).
SDS-PAGE Analysis of Purified HER-2 Mouse Monoclonal Antibody (HRB2/776). Confirmation of Integrity and Purity of Antibody.
Immunofluorescent staining of MeOH-fixed MCF-7 cells with HER-2 Mouse Monoclonal Antibody (HRB2/776) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).
Immunofluorescent staining of PFA-fixed MCF-7 cells with HER-2 Mouse Monoclonal Antibody (HRB2/776) followed by goat anti-mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).
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