Mitochondria (Marker for Human Cells, Granular RCC s & Salivary Tumor Antibody, IgG1, Clone: [113-1], Mouse, Monoclonal
Artikelnummer:
NBT-MSM1-739-P0
Hersteller Artikelnummer:
MSM1-739-P0
Alternativnummer:
NBT-MSM1-739-P0-20,NBT-MSM1-739-P0-100
Hersteller:
NeoBiotechnologies
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
IF, IHC, WB
Spezies Reaktivität:
Human
Immunogen:
Semi-Purified mitochondrial preparation
Alternative Synonym:
Not Known
This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial MAb has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Reportedly, this MAb facilitates the classification of salivary tumors.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[113-1]
Molekulargewicht:
60kDa
Isotyp:
IgG1
NCBI:
Not Known
Formulierung:
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml),Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&d
Western Blot analysis
Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Mitochondria Mouse Monoclonal Antibody (113-1).
Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Mouse Monoclonal Antibody (113-1).
Western Blot analysis of HeLa cell lysate using Mitochondria Mouse Monoclonal Antibody (113-1).
Confocal immunofluorescence image of HeLa cells using Mitochondria Mouse Monoclonal Antibody (113-1) labeled is Green (CF488) and Reddot is used to label the nuclei Red.
SDS-PAGE Analysis Purified Mitochondria Mouse Monoclonal Antibody (113-1). Confirmation of Integrity and Purity of Antibody.
Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Mitochondria Mouse Monoclonal Antibody (113-1).
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