A synthetic peptide of 15 amino acid from the C-terminus of human keratin 14.
Alternative Names:
CK-14, Dowling Meara, ebs3, ebs4, Epidermolysis Bullosa Simplex, k14, Keratin 14, Keratin Type I Cytoskeletal 14, Koebner, krt14, NFJ
Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 9
SDS-PAGE Analysis Puri
Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin 14 Mouse Monoclonal Antibody (LL002).
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Mouse Monoclonal Antibody (LL002).
SDS-PAGE Analysis Purified Cytokeratin 14 Mouse Monoclonal Antibody (LL002). Confirmation of Purity and Integrity of Antibody.
Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Mouse Monoclonal Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).
Flow Cytometric Analysis of trypsinized MeOH-fixed HeLa cells using Cytokeratin 14 Mouse Monoclonal Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).
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