TDP-43 Recombinant Mouse Monoclonal Antibody, Clone: [G6A6], Unconjugated
Catalog Number:
BYT-ORB1173017
- Images (6)
| Article Name: | TDP-43 Recombinant Mouse Monoclonal Antibody, Clone: [G6A6], Unconjugated |
| Biozol Catalog Number: | BYT-ORB1173017 |
| Supplier Catalog Number: | orb1173017 |
| Alternative Catalog Number: | BYT-ORB1173017-25,BYT-ORB1173017-50,BYT-ORB1173017-100 |
| Manufacturer: | Biorbyt |
| Host: | Mouse |
| Category: | Antikörper |
| Application: | ICC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Conjugation: | Unconjugated |
| Alternative Names: | ALS10, TDP-43, 1190002A23Rik, Tdp43, TADBP_HUMAN, TARDBP, TADBP_MOUSE, |
| TDP-43 Recombinant Mouse Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [G6A6] |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | TARDBP |
| Application Dilute: | WB=1:1000-5000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500 |
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Blocking buffer: 5% NFDM/TBST, Primary Ab Dilution: 1:5000, Primary Ab incubation condition: 4C overnight, Secondary Ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: 1: HeLa, 2: Jurkat, 3: HepG2, 4: NIH/3T3, Protein loading quantity: 20 µg, Exposure time: 60 s, Predicted MW: 45 kDa, Observed MW: 43 kDa. |
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Cell line: HeLa, Fixative: 100% Ice-cold methanol, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:50, Primary incubation condition: 4C overnight, Secondary Ab: Goat Anti-Mouse IgG, Nuclear counter stain: DAPI (Blue), Counter stain: Tubulin (Red), Comment: Color green is the positive signal for orb1173017. |
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Tissue: Human cervix cancer, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173017. |
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Tissue: Human spleen, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173017. |
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Tissue: Mouse brain, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173017. |
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Tissue: Rat brain, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary Ab Dilution: 1:100, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173017. |
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