FZD3 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB156979
- Images (6)
| Article Name: | FZD3 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB156979 |
| Supplier Catalog Number: | orb156979 |
| Alternative Catalog Number: | BYT-ORB156979-50,BYT-ORB156979-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, WB |
| Species Reactivity: | Human, Rat |
| Immunogen: | Recombinant human FZD3 protein (23-205/666aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | Fz-3, D930050A07Rik, Fz3, mFz3, FZD3_HUMAN, FZD3, hFz3, FZD3_MOUSE, |
| FZD3 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 74 kDa |
| UniProt: | Q9NPG1 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | FZD3 |
| Application Dilute: | WB=1:500-1000, ELISA=1:5000-10000 |
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Protein: brain (Rat) lysates at 30 ug, Primary: Anti-FZD3 (orb156979) at 1:300, Secondary: HRP conjugated Goat Anti-Rabbit IgG (orb572747) at 1:3000, ECL excitated the fluorescence, Predicted band size: 74kD, Observed band size: 74kD, 25kD. |
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Protein: pancreas (mouse) lysates at 30 ug, Primary: Anti-FZD3 (orb156979) at 1:300, Secondary: HRP conjugated Goat-Anti-Rabbit IgG (orb572747) at 1:5000, ECL excitated the fluorescence, Predicted band size: 74kD, Observed band size: 74kD. |
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Sample: Lane 1: Human SW480 cell Lysates, Lane 2: Human U-2 OS cell Lysates, Lane 3: Human A431 cell Lysates, Lane 4: Human SH-SY5Y cell Lysates, Primary: Anti-FZD3 (orb156979) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 74kDa, Observed band size: 70kDa. |
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Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-FZD3 Polyclonal Antibody, Unconjugated (orb156979) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-FAD3 Polyclonal Antibody, Unconjugated (orb156979) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-FZD3 Polyclonal Antibody, Unconjugated (orb156979) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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