APEX1 Antibody - middle region

Catalog Number: ASB-ARP58028_P050
Article Name: APEX1 Antibody - middle region
Biozol Catalog Number: ASB-ARP58028_P050
Supplier Catalog Number: ARP58028_P050
Alternative Catalog Number: ASB-ARP58028_P050-25UL,ASB-ARP58028_P050-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: IHC, WB
Species Reactivity: Bovine, Canine, Equine, Goat, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish
Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human APEX1
Alternative Names: APE, APX, APE1, APEN, APEX, HAP1, REF1
Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5 to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene, all encode the same protein.
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 36 kDa
NCBI: 328
UniProt: P27695
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.
Rabbit Anti-APEX1 Antibody
Catalog Number: ARP58028_P050
Formalin Fixed Paraffin Embedded Tissue: Human Liver Tissue
Observed Staining: Cytoplasm in hepatocytes
Primary Antibody Concentration: N/A
Other Working Concentrations: 1:600
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
WB Suggested Anti-APEX1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: 293T cell lysateAPEX1 is supported by BioGPS gene expression data to be expressed in HEK293T
APEX1 antibody - middle region (ARP58028_P050) in Human 293T using Western Blot