BAX Antibody - N-terminal region

Catalog Number: ASB-ARP58870_P050
Article Name: BAX Antibody - N-terminal region
Biozol Catalog Number: ASB-ARP58870_P050
Supplier Catalog Number: ARP58870_P050
Alternative Catalog Number: ASB-ARP58870_P050-25UL,ASB-ARP58870_P050-100UL
Manufacturer: Aviva
Host: Rabbit
Category: Proteine/Peptide
Application: IHC, WB
Species Reactivity: Bovine, Canine, Equine, Guinea pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep
Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human BAX
Alternative Names: BCL2L4
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
Clonality: Polyclonal
Concentration: 0.5 mg/ml
Molecular Weight: 24kDa
NCBI: 581
UniProt: Q07812
Form: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Rabbit Anti-BAX Antibody
Catalog Number: ARP58870_P050
Formalin Fixed Paraffin Embedded Tissue: Human Lymph Node Tissue
Observed Staining: Cytoplasm
Primary Antibody Concentration: 1:100
Other Working Concentrations: N/A
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 - 2.0 sec
WB Suggested Anti-BAX Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: HepG2 cell lysate
Researcher: Rosa Carotenuto, Department of Structural and Functional Biology, University of Naples Federico II
Application: Western blotting
Species + Tissue/Cell type: xenopus laevis embryos
How many ugs of tissue/cell lysate run on the gel: 1. 50 ug xenopus laevis embryos lysate (lysate buffer) 2. 50 ug xenopus laevis embryos lysate (HB buffer)
Primary antibody dilution: 1:400
Secondary antibody: Goat Anti-Rabbit AP
Secondary antibody dilution: 1:20000
BAX antibody - N-terminal region (ARP58870_P050) in Human HepG2 using Western Blot