Rabbit CD44 Recombinant Monoclonal Antibody, Clone: [BLR038F], Unconjugated
Catalog Number:
BYT-ORB1519905
- Images (7)
| Article Name: | Rabbit CD44 Recombinant Monoclonal Antibody, Clone: [BLR038F], Unconjugated |
| Biozol Catalog Number: | BYT-ORB1519905 |
| Supplier Catalog Number: | orb1519905 |
| Alternative Catalog Number: | BYT-ORB1519905-10,BYT-ORB1519905-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, ICC, IHC, IP, WB |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Between 692 and C-terminus |
| Conjugation: | Unconjugated |
| Alternative Names: | CD antigen CD44, CD44 antigen, CDW44, cell surface glycoprotein CD44, chondroitin sulfate proteoglycan 8, CSPG8, ECMR-III, epican, extracellular matrix receptor III, GP90 lymphocyte homing/adhesion receptor, HCELL, hematopoietic cell E- and L-selectin ligand, heparan sulfate proteoglycan, Hermes antigen, homing function and Indian blood group system, HUTCH-I, hyaluronate receptor, IN, Indian blood group antigen, LHR, MC56, MDU2, MDU3, MIC4, Pgp1, PGP-1, PGP-I, phagocytic glycoprotein 1, Phagocytic glycoprotein I, soluble CD44 |
| CD44 Antibody |
| Application Dilute: | WB - 1:1000, IP - 20 ul/1mg lysate, IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections., ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
| Application Notes: | Application Notes: Format: Whole IgG |
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Detection of mouse CD44 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG. |
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Detection of human CD44 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG. |
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Detection of human CD44 in FFPE 786-O cells by ICC. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB. |
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Detection of human CD44 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB. |
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Detection of human CD44 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction, 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) used for IP at 20 µl/mg lysate. |
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Detection of human CD44 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, 786-O, HEK293T, A-549, and KG-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody. |
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Detection of mouse CD44 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody. |
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