CPLX1 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB156445
Article Name: CPLX1 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB156445
Supplier Catalog Number: orb156445
Alternative Catalog Number: BYT-ORB156445-50,BYT-ORB156445-100,BYT-ORB156445-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human CPLX1 (31-100/134aa)
Conjugation: Unconjugated
Alternative Names: CPX-I, CPX1, DEE63, EIEE63, 921-S, CPLX1_BOVIN, CPLX1, CPLX1_HUMAN, Complexin I (CPX I), Synaphin-2, CPLX1_MOUSE, CPLX1_RAT,
CPLX1 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 15 kDa
UniProt: O14810
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: CPLX1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Immunohistochemical staining of rat brain tissue using CPLX1 antibody.
Immunohistochemical staining of Mouse brain tissue using CPLX1 antibody.
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CPLX1) Polyclonal Antibody, Unconjugated (orb156445) at 1:400 overnight at 4C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37C, followed by a conjugated streptavidin at 1:500 for 40 minutes and DAPI staining of the nuclei.
Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CPLX1) Polyclonal Antibody, Unconjugated (orb156445) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CPLX1) Polyclonal Antibody, Unconjugated (orb156445) at 1:400 overnight at 4C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20 minutes at 37C, followed by a conjugated streptavidin at 1:500 for 40 minutes and DAPI staining of the nuclei.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (CPLX1) Polyclonal Antibody, Unconjugated (orb156445) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: RSC96 (Rat) Cell Lysate at 30 ug, Primary: Anti-CPLX1 (orb156445) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 15 kD, Observed band size: 17 kD.



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