E cadherin Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB156677
- Images (7)
| Article Name: | E cadherin Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB156677 |
| Supplier Catalog Number: | orb156677 |
| Alternative Catalog Number: | BYT-ORB156677-50,BYT-ORB156677-100,BYT-ORB156677-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human E-cadherin (401-500/882aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO, E-cad, L-CAM, Um, Cadherin-1, Uvomorulin, CADH1_BOVIN, CDH1, Epithelial cadherin (E-cadherin), CADH1_HUMAN, CAM 120/80, CADH1_MOUSE, CADH1_RAT, |
| E cadherin Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 90/97 kDa |
| UniProt: | P12830 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | CDH1 |
| Application Dilute: | WB=1:1000-5000, IHC-P=1:200-800, IHC-F=1:200-800, IF=1:200-800 |
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Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (E cadherin) Polyclonal Antibody, Unconjugated (orb156677) at 1:400 overnight at 4C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (E cadherin) Polyclonal Antibody, Unconjugated (orb156677) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human stomach), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (E cadherin) Polyclonal Antibody, Unconjugated (orb156677) at 1:400 overnight at 4C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (E cadherin) Polyclonal Antibody, Unconjugated (orb156677) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (E cadherin) Polyclonal Antibody, Unconjugated (orb156677) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: HT29 (Human) Cell Lysate at 30 ug, PC-3 (Human) Cell Lysate at 30 ug, Primary: Anti-E cadherin (orb156677) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 20/80 kD, Observed band size: 130 kD. |
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Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: A549 (Human) Cell Lysate at 30 ug, Lane 3: HepG2 (Human) Cell Lysate at 30 ug, Primary: Anti-E cadherin (orb156677) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 125 kD, Observed band size: 125 kD. |
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