FLNC Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB156880
- Images (7)
| Article Name: | FLNC Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB156880 |
| Supplier Catalog Number: | orb156880 |
| Alternative Catalog Number: | BYT-ORB156880-50,BYT-ORB156880-100,BYT-ORB156880-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Filamin 2 (251-350/2725aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | ABP-280, ABP280A, ABPA, ABPL, ARVC15, CMH26, FLN2, MFM5, MPD4, RCM5, 1110055E19Rik, ABP-L, FLN-C, FLNC_HUMAN, FLNC, ABP-280-like protein, Actin-binding-like protein, Filamin-2, Gamma-filamin, FLNC_MOUSE, |
| FLNC Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 300 kDa |
| UniProt: | Q14315 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | FLNC |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Immunohistochemical staining of rat cardiac muscle tissue using Filamin 2 antibody. |
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Immunohistochemical staining of rat cardiac muscle tissue using Filamin 2 antibody. |
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Paraformaldehyde-fixed, paraffin embedded (Mouse heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (FLNC) Polyclonal Antibody, Unconjugated (orb156880) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (FLNC) Polyclonal Antibody, Unconjugated (orb156880) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Human U-2 OS cell lysates, Primary: Anti-FLNC (orb156880) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 300 kDa, Observed band size: 280 kDa. |
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Tissue/Cell: rat cardiac muscle, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-FLNC Polyclonal Antibody, Unconjugated (orb156880) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: rat cardiac muscle, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-FLNC Polyclonal Antibody, Unconjugated (orb156880) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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