Phospho-FMRP (Ser500) Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB156909
- Images (7)
| Article Name: | Phospho-FMRP (Ser500) Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB156909 |
| Supplier Catalog Number: | orb156909 |
| Alternative Catalog Number: | BYT-ORB156909-50,BYT-ORB156909-100,BYT-ORB156909-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Mouse, Rat |
| Immunogen: | KLH conjugated synthesised phosphopeptide derived from human FMRP around the phosphorylation site of Ser500 NA(p-S)ET |
| Conjugation: | Unconjugated |
| Alternative Names: | FMR1 (p-S500), p-FMR1, phospho-FMR1, FMRP, FRAXA, POF, POF1, FMR1_HUMAN, FMR1, Fragile X messenger ribonucleoprotein (FMRP), Protein FMR-1, |
| Phospho-FMRP (Ser500) Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 75 kDa |
| UniProt: | Q06787 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | FMR1 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
| Application Notes: | Modification: Phosphorylated |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-FMRP (Ser500)) Polyclonal Antibody, Unconjugated (orb156909) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-FMRP (Ser500)) Polyclonal Antibody, Unconjugated (orb156909) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-FMRP (Ser500)) Polyclonal Antibody, Unconjugated (orb156909) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (phospho-FMRP (Ser500)) Polyclonal Antibody, Unconjugated (orb156909) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Protein: brain (mouse) lysates at 40 ug, Primary: Anti-P-FMRP (orb156909) at 1:300, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:5000, ECL excitated the fluorescence, Predicted band size: 75 kD, Observed band size: 75 kD. |
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Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-FMRP (Ser500) Polyclonal Antibody, Unconjugated (orb156909) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-phospho-FMRP (Ser500) Polyclonal Antibody, Unconjugated (orb156909) 1:200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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