Gemin 2 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB157105
Article Name: Gemin 2 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB157105
Supplier Catalog Number: orb157105
Alternative Catalog Number: BYT-ORB157105-50,BYT-ORB157105-100,BYT-ORB157105-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Rat
Immunogen: KLH conjugated synthetic peptide derived from human Gemin 2 (101-200/280aa)
Conjugation: Unconjugated
Alternative Names: SIP1, SIP1-delta, gemin-2, GEMI2_HUMAN, GEMIN2, Component of gems 2, Survival of motor neuron protein-interacting protein 1 (SMN-interacting protein 1), GEMI2_MOUSE, GEMI2_RAT,
Gemin 2 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 32 kDa
UniProt: O14893
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: GEMIN2
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Gemin 2) Polyclonal Antibody, Unconjugated (orb157105) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Gemin 2) Polyclonal Antibody, Unconjugated (orb157105) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat spinal cord), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Gemin 2) Polyclonal Antibody, Unconjugated (orb157105) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Gemin 2) Polyclonal Antibody, Unconjugated (orb157105) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat thymus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Gemin 2) Polyclonal Antibody, Unconjugated (orb157105) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: HepG2 (Human) Cell Lysate at 30 ug, MCF-7 (Human) Cell Lysate at 30 ug, Primary: Anti-Gemin 2 (orb157105) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 32 kD, Observed band size: 32 kD.
IHC-P analysis of rat testis using Gemin 2 Polyclonal Antibody at 1:200 dilution.