Glutaredoxin 2 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB157191
- Images (7)
| Article Name: | Glutaredoxin 2 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB157191 |
| Supplier Catalog Number: | orb157191 |
| Alternative Catalog Number: | BYT-ORB157191-50,BYT-ORB157191-100,BYT-ORB157191-200 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P |
| Species Reactivity: | Human, Rat |
| Immunogen: | KLH conjugated synthetic peptide derived from human Glutaredoxin 2 (101-164/164aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | CGI-133, GRX2, 1700010P22Rik, CR201_G0024230, GLRX2_BOVIN, GLRX2, GLRX2_HUMAN, GLRX2_MOUSE, GLRX2_PONAB, GLRX2_RAT, |
| Glutaredoxin 2 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 16 kDa |
| UniProt: | Q9NS18 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | LRX2 |
| Application Dilute: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test |
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Immunohistochemical staining of rat brain tissue using Glutaredoxin 2 antibody. |
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Immunohistochemical staining of human colon carcinoma tissue using Glutaredoxin 2 antibody. |
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Blank control (blue line): RSC96 (fixed with pre-warmed 4% paraformaldehyde for 30 min at 37C and then permeabilized with 90% ice-cold methanol for 30 min on ice), Primary Antibody (green line): Rabbit Anti-Glutaredoxin 2 antibody (orb157191), Dilution: 0.2 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GLRX2) Polyclonal Antibody, Unconjugated (orb157191) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GLRX2) Polyclonal Antibody, Unconjugated (orb157191) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (GLRX2) Polyclonal Antibody, Unconjugated (orb157191) at 1:500 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining. |
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Tissue/Cell: Rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37C for 20 min, Incubation: Anti-GLRX2 Polyclonal Antibody, Unconjugated (orb157191) 1:500, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining. |
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