MCT1 Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB157860
Article Name: MCT1 Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB157860
Supplier Catalog Number: orb157860
Alternative Catalog Number: BYT-ORB157860-50,BYT-ORB157860-100,BYT-ORB157860-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human MCT1 (251-350/500aa)
Conjugation: Unconjugated
Alternative Names: HHF7, MCT, MCT1, MCT1D, MOT1_HUMAN, SLC16A1, MCT 1, Solute carrier family 16 member 1, MOT1_MOUSE,
MCT1 Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 55 kDa
UniProt: P53985
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: SLC16A1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1µg/Test
Flow cytometric analysis of MCF7 cell using MCT1 antibody.
Western blot analysis of HepG2 Human cell Lysate using MCT1 antibody.
Blank control: K562. Primary Antibody (green line): Rabbit Anti-MCT1 antibody (orb157860), Dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Blank control: MCF7 Cells (blue). Primary Antibody: Rabbit Anti-MCT1/AF647 Conjugated antibody, Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/FITC (orange), used under the same conditions.
Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MCT1) Polyclonal Antibody, Unconjugated (orb157860) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: HepG2 (Human) cell Lysate at 40 ug, Primary: Anti-MCT1 (orb157860) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 55kD, Observed band size: 55kD.
Sample: Lane 1: Liver (Mouse) Lysate at 40 ug, Lane 2: Stomach (Mouse) Lysate at 40 ug, Lane 3: Heart (Mouse) Lysate at 40 ug, Lane 4: Liver (Rat) Lysate at 40 ug, Lane 5: Stomach (Rat) Lysate at 40 ug, Lane 6: Heart (Rat) Lysate at 40 ug, Primary: Anti-MCT1 (orb157860) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48 kD, Observed band size: 48 kD.



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