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Paraformaldehyde-fixed, paraffin embedded (human pancreatic carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MUC5AC) Polyclonal Antibody, Unconjugated (orb157913) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MUC5AC) Polyclonal Antibody, Unconjugated (orb157913) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human stomach), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (MUC5AC) Polyclonal Antibody, Unconjugated (orb157913) at 1:400 overnight at 4C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse stomach tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MUC5AC) Polyclonal Antibody, Unconjugated (orb157913) at 1:400 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (rat stomach), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (MUC5AC) Polyclonal Antibody, Unconjugated (orb157913) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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IHC-P analysis of Human stomach using MUC5AC Polyclonal Antibody at 1:400 dilution. |
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IHC-P analysis of rat stomach using MUC5AC Polyclonal Antibody at 1:200 dilution. |