TAZ Rabbit Polyclonal Antibody, Unconjugated

Catalog Number: BYT-ORB158573
Article Name: TAZ Rabbit Polyclonal Antibody, Unconjugated
Biozol Catalog Number: BYT-ORB158573
Supplier Catalog Number: orb158573
Alternative Catalog Number: BYT-ORB158573-50,BYT-ORB158573-100,BYT-ORB158573-200
Manufacturer: Biorbyt
Host: Rabbit
Category: Antikörper
Application: FC, IF, IHC-Fr, IHC-P, WB
Species Reactivity: Human, Mouse, Rat
Immunogen: KLH conjugated synthetic peptide derived from human TAZ (1-100/400aa)
Conjugation: Unconjugated
Alternative Names: TAZ, 2310058J06Rik, 2610021I22Rik, WWTR1_HUMAN, WWTR1, Transcriptional coactivator with PDZ-binding motif, WWTR1_MOUSE,
TAZ Rabbit Polyclonal Antibody
Clonality: Polyclonal
Concentration: 1mg/ml
Molecular Weight: 44 kDa
Pubmed: 25029906
UniProt: Q9GZV5
Buffer: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Form: Liquid
Target: WWTR1
Application Dilute: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=3ug/test
Blank control: A549. Primary Antibody (green line): Rabbit Anti-TAZ antibody (orb158573), dilution: 1 µg/10 6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 3 µg/Test. Protocol The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (mouse placenta), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TAZ) Polyclonal Antibody, Unconjugated (orb158573) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (TAZ) Polyclonal Antibody, Unconjugated (orb158573) at 1:400 overnight at 4C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug, Lane 2: Kidney (Rat) Lysate at 40 ug, Primary: Anti-TAZ (orb158573) at 1/1000 dilution, Anti-beta-Actin (orb500817) at 1/2000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50-55 kD, Observed band size: 52 kD.
Sample: MCF-7 (Human) Cell Lysate at 40 ug, Lovo (Human) Cell Lysate at 40 ug, A549 (Human) Cell Lysate at 40 ug, Primary: Anti-TAZ (orb158573) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 44kD, Observed band size: 46kD.
Immunohistochemical staining of rat heart tissue using TAZ antibody.
Immunohistochemical staining of rat heart tissue using TAZ antibody.



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