Bcl-2 Recombinant Rabbit Monoclonal Antibody, Clone: [B9E6], Unconjugated
Catalog Number:
BYT-ORB2563019
- Images (8)
| Article Name: | Bcl-2 Recombinant Rabbit Monoclonal Antibody, Clone: [B9E6], Unconjugated |
| Biozol Catalog Number: | BYT-ORB2563019 |
| Supplier Catalog Number: | orb2563019 |
| Alternative Catalog Number: | BYT-ORB2563019-100,BYT-ORB2563019-25,BYT-ORB2563019-50 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse |
| Immunogen: | A synthesized peptide derived from human Bcl 2 (1-239/239aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | Bcl-2, PPP1R50, BCL2_HUMAN, BCL2, |
| Bcl-2 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [B9E6] |
| Molecular Weight: | 26 kDa |
| UniProt: | P10415 |
| Buffer: | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | BCL2 |
| Application Dilute: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, WB=1:500-2000 |
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25 ug total protein per lane of various lysates probed with Bcl-2 monoclonal antibody, unconjugated (orb2563019) at 1:2000 dilution and 4C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min. |
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Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Human Lymph, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Mouse Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Bcl-2 Monoclonal Antibody, Unconjugated (orb2563019) at 1:200 overnight at 4C, followed by conjugation to the SP Kit (Rabbit) and DAB staining. |
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