PARP1 Rabbit Polyclonal Antibody, Unconjugated
Catalog Number:
BYT-ORB704256
- Images (8)
| Article Name: | PARP1 Rabbit Polyclonal Antibody, Unconjugated |
| Biozol Catalog Number: | BYT-ORB704256 |
| Supplier Catalog Number: | orb704256 |
| Alternative Catalog Number: | BYT-ORB704256-50,BYT-ORB704256-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | ELISA, ICC, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Recombinant human PARP1 (81-390/1014aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1, 5830444G22Rik, Adprp, msPARP, sPARP-1, PARP1_HUMAN, PARP1, ADP-ribosyltransferase diphtheria toxin-like 1 (ARTD1), DNA ADP-ribosyltransferase PARP1, NAD(+) ADP-ribosyltransferase 1 (ADPRT 1), Poly[ADP-ribose] synthase 1, Protein poly-ADP-ribosyltransferase PARP1, 2.4.2.30, PARP1_MOUSE, Poly[ADP-ribose] synthase 1 (msPARP), PARP1_RAT, |
| PARP1 Rabbit Polyclonal Antibody |
| Clonality: | Polyclonal |
| Concentration: | 1mg/ml |
| Molecular Weight: | 113 kDa |
| UniProt: | P09874 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | PARP1 |
| Application Dilute: | WB=1:500-2000, ICC/IF=1:50-200, ELISA=1:5000-10000 |
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C6 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (KO Validated) PARP polyclonal Antibody, Unconjugated (orb704256) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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NIH/3T3 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (KO Validated) PARP polyclonal Antibody, Unconjugated (orb704256) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (PARP) Polyclonal Antibody, Unconjugated (orb704256) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: 293T (Human) Cell Lysate at 30 ug, Lane 2: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-PARP (orb704256) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 110 kD, Observed band size: 110 kD. |
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Sample: Lane 1: Hela (Human) Cell Lysate at 25 ug, Lane 2: PARP1 knockout (KO) Hela (Human) Cell Lysate at 25 ug, Primary: Anti-PARP1 (orb704256) at 1/1000 dilution, Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution, Predicted band size: 110 kD, Observed band size: 110 kD. |
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U2-OS cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (KO Validated) PARP polyclonal Antibody, Unconjugated (orb704256) 1:100, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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U2-OS cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (KO Validated) PARP polyclonal Antibody, Unconjugated (orb704256) 1:200, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Western blot:Lane 1:293T (Human) Cell,Lane 2:U251 (Human) Cell,Primary:Anti-PARP at 1:1000 dilution. |
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