BST2 Recombinant Rabbit Monoclonal Antibody, Clone: [1C3], Unconjugated
Catalog Number:
BYT-ORB704295
- Images (8)
| Article Name: | BST2 Recombinant Rabbit Monoclonal Antibody, Clone: [1C3], Unconjugated |
| Biozol Catalog Number: | BYT-ORB704295 |
| Supplier Catalog Number: | orb704295 |
| Alternative Catalog Number: | BYT-ORB704295-50,BYT-ORB704295-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human |
| Immunogen: | KLH conjugated synthetic peptide derived from human BST2 |
| Conjugation: | Unconjugated |
| Alternative Names: | CD317, HM1.24, TETHERIN, BST2_HUMAN, BST2, BST-2, HM1.24 antigen, |
| BST2 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [1C3] |
| Molecular Weight: | 18 kDa |
| UniProt: | Q10589 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | BST2 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:100-500, Flow-Cyt=1:50-200 |
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Blank control: Jurkat. Primary Antibody (green line): Rabbit Anti-BST2 antibody (orb704295), dilution: 1:50, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1:1000. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (BST2) Monoclonal Antibody, Unconjugated (orb704295) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human colon cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (BST2) Monoclonal Antibody, Unconjugated (orb704295) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (BST2) Monoclonal Antibody, Unconjugated (orb704295) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (BST2) Monoclonal Antibody, Unconjugated (orb704295) at 1:50 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: Hela cell lysate, Lane 2: Siha cell lysatee, Primary: Anti-BST2 (orb704295) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 18 kD, Observed band size: 40 kD. |
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IHC-P analysis of human colon cancer using BST2 Monoclonal Antibody at 1:50 dilution. |
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Western blot:Lane 1:Hela Cell,Lane 2:Siha Cell,Primary:Anti-BST2 at 1:500 dilution. |
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