Collagen I Recombinant Rabbit Monoclonal Antibody, Clone: [5F2], Unconjugated
Catalog Number:
BYT-ORB704339
- Images (8)
| Article Name: | Collagen I Recombinant Rabbit Monoclonal Antibody, Clone: [5F2], Unconjugated |
| Biozol Catalog Number: | BYT-ORB704339 |
| Supplier Catalog Number: | orb704339 |
| Alternative Catalog Number: | BYT-ORB704339-50,BYT-ORB704339-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | FC, IF, IHC-Fr, IHC-P |
| Species Reactivity: | Human |
| Immunogen: | KLH conjugated synthetic peptide derived from human Collagen I |
| Conjugation: | Unconjugated |
| Alternative Names: | CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4, Col1a-1, Cola-1, Cola1, Mov-13, Mov13, CO1A1_BOVIN, COL1A1, Alpha-1 type I collagen, CO1A2_CANLF, COL1A2, Alpha-2 type I collagen, |
| Collagen I Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [5F2] |
| Molecular Weight: | 130 kDa |
| UniProt: | P02452 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | COL1A1 |
| Application Dilute: | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1:50-100 |
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Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Collagen I) Monoclonal Antibody, Unconjugated (orb704339) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Collagen I) Monoclonal Antibody, Unconjugated (orb704339) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Collagen I) Monoclonal Antibody, Unconjugated (orb704339) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human prostate), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Collagen I) Monoclonal Antibody, Unconjugated (orb704339) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (human prostate), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Collagen I) Monoclonal Antibody, Unconjugated (orb704339) at 1:200 overnight at 4C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human skin cancer), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Antibody incubation with (Collagen I) Monoclonal Antibody, Unconjugated (orb704339) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Sample: Lane 1: human placenta tissue lysates, Primary: Anti-Collagen I (orb704339) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 130 kD, Observed band size: 130 kD. |
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IHC-P analysis of human kidney tissue using Collagen I Monoclonal Antibody at 1:50 dilution. |
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