NMDAR1 Recombinant Rabbit Monoclonal Antibody, Clone: [13C4], Unconjugated
Catalog Number:
BYT-ORB704351
- Images (8)
| Article Name: | NMDAR1 Recombinant Rabbit Monoclonal Antibody, Clone: [13C4], Unconjugated |
| Biozol Catalog Number: | BYT-ORB704351 |
| Supplier Catalog Number: | orb704351 |
| Alternative Catalog Number: | BYT-ORB704351-25,BYT-ORB704351-50,BYT-ORB704351-100 |
| Manufacturer: | Biorbyt |
| Host: | Rabbit |
| Category: | Antikörper |
| Application: | IF, IHC-Fr, IHC-P, WB |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | A synthesized peptide derived from human GluN1 (850-900aa) |
| Conjugation: | Unconjugated |
| Alternative Names: | DEE101, GluN1, MRD8, NDHMSD, NDHMSR, NMD-R1, NMDA1, NMDAR1, NR1, hNR1, GluRdelta1, GluRzeta1, M100174, Nmdar, Rgsc174, NMDZ1_HUMAN, GRIN1, Glutamate [NMDA] receptor subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1 (NMD-R1 | hNR1), NMDZ1_MOUSE, N-methyl-D-aspartate receptor subunit NR1 (NMD-R1), Glurz1, NMDZ1_RAT, |
| NMDAR1 Recombinant Rabbit Monoclonal Antibody |
| Clonality: | Recombinant |
| Concentration: | 1mg/ml |
| Clone Designation: | [13C4] |
| Molecular Weight: | 103 kDa |
| UniProt: | Q05586 |
| Buffer: | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Form: | Liquid |
| Target: | GRIN1 |
| Application Dilute: | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 |
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Blank control: SH-SY5Y. Primary Antibody (green line): Rabbit Anti-NMDAR1 antibody (orb704351), dilution: 1:50, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1:1000. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed. |
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N2A cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (NMDAR1) monoclonal Antibody, Unconjugated (orb704351) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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Paraformaldehyde-fixed, paraffin embedded (human cerebellum), Antigen retrieval by boiling in EDTA buffer buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NMDAR1) Monoclonal Antibody, Unconjugated (orb704351) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (Human left parietal lobe), Antigen retrieval by boiling in EDTA buffer buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NMDAR1) Monoclonal Antibody, Unconjugated (orb704351) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in EDTA buffer buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NMDAR1) Monoclonal Antibody, Unconjugated (orb704351) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum), Antigen retrieval by boiling in EDTA buffer buffer (pH9.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37C for 30 min, Incubation with (NMDAR1) Monoclonal Antibody, Unconjugated (orb704351) at 1:200 overnight at 4C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
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SHG-44 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (NMDAR1) monoclonal Antibody, Unconjugated (orb704351) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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SH-SY5Y cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37C for 20 min, Antibody incubation with (NMDAR1) monoclonal Antibody, Unconjugated (orb704351) 1:50, 90 minutes at 37C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37C for 90 minutes, DAPI (blue) was used to stain the cell nuclei. |
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