A phospho specific peptide corresponding to the amino acids surrounding tyrosine 72 of mouse CD3 zeta linked to KLH
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta (CD247). These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Clonality:
Monoclonal
Concentration:
1 mg/ml
Clone Designation:
[EM-26]
Isotype:
Mouse IgG2b
Buffer:
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage:
2°C to 8°C
Target:
CD3 zeta (pY72)
Antibody Type:
Monoclonal Antibody
Application Notes:
Western blotting: Recommended dilution: 2 - 5 µg/ml, positive control: Jurkat cells lysate treated with pervanadate, splenocyte lysate of Balb/c or F1 mouse treated with pervanadate, non-reducing conditions recommended. Flow cytometry: Intracellular staining, recommended dilution: 1-9 µg/ml, positive control: Jurkat cells treated with pervanadate, T-cells from lymph nodes of OT-1 mouse treated with pervanadate.
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