This MAb recognizes a protein of 130kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels.Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.
200ug/ml of recombinant MAb Purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Western Blot (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95
Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Recombinant Mouse Monoclonal Antibody (rCDH16/1071).
SDS-PAGE Analysis of Purified KSP-Cadherin Recombinant Monoclonal Antibody (rCDH16/1071). Confirmation of Integrity and Purity of Antibody.
Western Blot Analysis of human kidney tissue lysate using KSP-Cadherin Recombinant Mouse Monoclonal Antibody (rCDH16/1071).
Flow Cytometric Analysis of Human HEK293 cells using KSP-Cadherin Recombinant Mouse Monoclonal Antibody (rCDH16/1071) followed by Goat anti-Mouse IgG-CF488 (Blue), Isotype Control (Red).
Western Blot Analysis of human kidney tissue lysate using KSP-Cadherin Recombinant Mouse Monoclonal Antibody (rCDH16/1071).
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