This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- OO OO induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal diluti
SDS-PAGE Analysis of Purified p27 Recombinant Rabbit Monoclonal Antibody (KIP1/1355R). Confirmation of Purity and Integrity of Antibody.
IHC analysis of formalin-fixed, paraffin-embedded human bladder. Strong nuclear staining using KIP1/1355R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
Western blot analysis of Human Skeletal Muscle, Mouse Skeletal Muscle, Rat Skeletal Muscle, Hamster Skeletal Muscle, Guinea pig Skeletal Muscle, and Rabbit Skeletal Muscle tissue lysates using p27 Recombinant Rabbit Monoclonal Antibody (KIP1/1355R).
Western Blot Analysis of NIH/3T3 cell lysate using p27 Recombinant Rabbit Monoclonal Antibody (KIP1/1355R).
Western Blot analysis of Human Spleen and Human Sk. Muscle tissue lysates using p27 Recombinant Rabbit Monoclonal Antibody (KIP1/1355R).
Western blot analysis of A431 lysate using p27 Recombinant Rabbit Monoclonal Antibody (KIP1/1355R).
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