Recognizes a protein of 57kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts and stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts and stromal cells stain. The histological differentiation of complete mole, partial mole, and hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease and choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei and stromal cells are labeled with this antibody. Similar findings apply to PHM and hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities and serve as an internal control.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
IHC analysis of formalin-fixed, paraffin-em
IHC analysis of formalin-fixed, paraffin-embedded human placenta. Strong nuclear staining using KIP2/7083R at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody, secondary only negative control.
IHC analysis of formalin-fixed, paraffin-embedded human kidney cancer. Strong nuclear staining using KIP2/7083R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
SDS-PAGE Analysis of Purified p57 Recombinant Rabbit Monoclonal Antibody (KIP2/7083R). Confirmation of Purity and Integrity of Antibody.
Western blot analysis of Human Brain tissue lysate using p57 Recombinant Rabbit Monoclonal Antibody (KIP2/7083R).
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