Collagen IV (COL4A1/COL4A2) Antibody, IgG1, Clone: [M3F7], Mouse, Monoclonal
Biozol Catalog Number:
NBT-1282-MSM3-P0
Supplier Catalog Number:
1282-MSM3-P0
Alternative Catalog Number:
NBT-1282-MSM3-P0-20,NBT-1282-MSM3-P0-100
Manufacturer:
NeoBiotechnologies
Host:
Mouse
Category:
Antikörper
Application:
FC, IF, IHC, WB
Species Reactivity:
Human
Immunogen:
alpha1 (IV)-alpha2(IV) triple helix. The epitope is triple helical domain about 900 from the N-terminus
Alternative Names:
Arresten, BSVD, COL4A1, COL4A1 NC1 domain, COL4A2, collagen alpha-1(IV) chain, Collagen IV Alpha 1 Polypeptide, Collagen IV Alpha 2 Polypeptide, Collagen Type IV Alpha 2, RATOR
Collagen Type IV is a major component of the basement membrane and plays an important role in cell adhesion, migration, differentiation and growth. Collagen Type IV express at the basement membranes in a variety of tissues including kidney, muscle, lymph nodes, lung, tendon and spleen. Collagen Type IV has been shown to be useful in differentiating microinvasive from in situ ductal carcinomas of the breast. Other Collagen Type IV studies include use in pancreatic adenocarcinoma and chronic pancreatitis, nephrosclerosis and other kidney diseases, oral squamous cell carcinoma, laryngeal cancers, ovarian cancers and cervical cancers. Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a chicken-wire meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin. M3F7 recognizes type IV collagen in basement membranes in kidney, lung, placenta, cornea and skin. This antibody does not recognize denatured type IV collagen.
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilutio
Formalin-fixed, paraffin-embedded human kidney adenocarcinoma. Strong staining of glomeruli using M3F7 at 2ug/ml in PBS for 30min RT. Inset: PBS instead of primary antibody, secondary negative control.
Formalin-fixed, paraffin-embeddedhuman kidney stained with Collagen IVMouse Monoclonal Antibody (M3F7).
Formalin-fixed, paraffin-embeddedhuman kidney stained with Collagen IVMouse Monoclonal Antibody (M3F7).
SDS-PAGE Analysis of Purified Collagen IV Mouse Monoclonal Antibody (COL4/6307). Confirmation of Purity and Integrity of Antibody
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