Collagen VII Antibody, IgG1, Clone: [LH7.2], Mouse, Monoclonal
Biozol Catalog Number:
NBT-1294-MSM1-P0
Supplier Catalog Number:
1294-MSM1-P0
Alternative Catalog Number:
NBT-1294-MSM1-P0-20,NBT-1294-MSM1-P0-100
Manufacturer:
NeoBiotechnologies
Host:
Mouse
Category:
Antikörper
Application:
ELISA, FC, IF, IHC, WB
Species Reactivity:
Bovine, Equine, Goat, Guinea pig, Human, Sheep
Immunogen:
Recombinant full-length human Collagen VII protein
Alternative Names:
Alpha 1 type VII collagen, Collagen 7, Collagen alpha 1(VII) chain, Collagen type VII alpha 1, Collagen VII alpha 1 polypeptide Col7a1, EBD1, EBDCT, EBR1, Long-chain (LC) collagen
Recognizes an epitope located on collagenase digested Type VII collagen (150kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
ELISA (For coating, order antibody without BSA), Western Blot (1-2ug/ml)Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires
SDS-PAGE Analysis of Purified Collagen VII
Formalin-fixed, paraffin-embedded human Skin stained with Collagen VII Mouse Monoclonal Antibody (LH7.2).
Formalin-fixed, paraffin-embedded human Skin stained with Collagen VII Mouse Monoclonal Antibody (LH7.2).
SDS-PAGE Analysis of Purified Collagen VII Mouse Monoclonal Antibody (LH7.2). Confirmation of Purity and Integrity of Antibody.
Immunofluorescence Analysis of PFA-fixed HeLa cells labeling Collagen VII using Collagen VII Mouse Monoclonal Antibody (LH7.2) followed by goat anti-mouse IgG-CF488 (Green). The nuclear counterstain is RedDot.
Flow Cytometric Analysis of PFA-fixed HeLa cells using Collagen VII Mouse Monoclonal Antibody (LH7.2) followed by goat anti-mouse IgG-CF488 (Blue), Isotype Control (Red).
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