Recombinant human full-length DES protein. Human Heart, Rat Heart, Hamster Heart, Guinea pig Heart, Rabbit Heart.
Alternative Names:
CMD1I, CSM1, CSM2, DES, Intermediate filament protein
Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain -helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles.Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilu
Formalin-fixed, paraffin-embedded human Skeletal Muscle stained with Desmin Rabbit Recombinant Monoclonal Antibody (DES/2960R).
SDS-PAGE Analysis of Purified Desmin Rabbit Recombinant Monoclonal Antibody (DES/2960R). Confirmation of Purity and Integrity of Antibody.
Western blot analysis of RH-30 cell lysate using Desmin Rabbit Recombinant Monoclonal Antibody (DES/2960R).
Western Blot Analysis of Human Heart, Mouse Heart, Rat Heart, Hamster Heart, Guinea pig Heart, and Rabbit Heart tissue lysates using Desmin Recombinant Rabbit Monoclonal Antibody (DES/2960R).
Western Blot Analysis of Human Heart tissue lysate using Desmin Recombinant Rabbit Monoclonal Antibody (DES/2960R).
Western Blot validation of anti-Desmin antibody. Desmin protein expression was compared in wild-type (WT) and Desmin shRNA Knockdown (KD) HT-1080 cells (30ug of total protein). Loading control is shown below.
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