Recognizes a protein (amino acid 482) of 55kDa (in vivo 110kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5-deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Recombinant Monoclonal Antibody
Application Notes:
Western Blot (2-4ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 min
Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Thymidine Phosphorylase Rabbit Recombinant Monoclonal (TYMP/2890R).
SDS-PAGE Analysis of Purified Thymidine Phosphorylase Rabbit Recombinant Monoclonal (TYMP/2890R). Confirmation of Purity and Integrity of Antibody.
Western Blot Analysis of human spleen tissue lysate using Thymidine Phosphorylase Rabbit Recombinant Monoclonal (TYMP/2890R).
Western Blot Analysis of Human Heart, Human Liver, Human Lung and Human Spleen tissue lysates using Thymidine Phosphorylase Recombinant Rabbit Monoclonal Antibody (TYMP/2890R).
Western Blot Analysis of Liver, Lung and Spleen tissue lysates of different species using Thymidine Phosphorylase Recombinant Rabbit Monoclonal Antibody (TYMP/2890R).
* VAT and and shipping costs not included. Errors and price changes excepted
