Mitochondria (Marker for Human Cells, Granular RCC s & Salivary Tumor Antibody, IgG1, Clone: [113-1], Mouse, Monoclonal
Biozol Catalog Number:
NBT-MSM1-739-P1ABX
Supplier Catalog Number:
MSM1-739-P1ABX
Alternative Catalog Number:
NBT-MSM1-739-P1ABX-100
Manufacturer:
NeoBiotechnologies
Host:
Mouse
Category:
Antikörper
Application:
IF, IHC, WB
Species Reactivity:
Human
Immunogen:
Semi-Purified mitochondrial preparation
Alternative Names:
Not Known
This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial MAb has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Reportedly, this MAb facilitates the classification of salivary tumors.
Clonality:
Monoclonal
Clone Designation:
[113-1]
Molecular Weight:
60kDa
Isotype:
IgG1
NCBI:
Not Known
Form:
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Immunofluorescence (1-2ug/ml), Western Blot (1-2ug/ml),Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&d
Western Blot analysis
Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Mitochondria Mouse Monoclonal Antibody (113-1).
Formalin-fixed, paraffin-embedded human Pancreas stained with Mitochondria Mouse Monoclonal Antibody (113-1).
Western Blot analysis of HeLa cell lysate using Mitochondria Mouse Monoclonal Antibody (113-1).
Confocal immunofluorescence image of HeLa cells using Mitochondria Mouse Monoclonal Antibody (113-1) labeled is Green (CF488) and Reddot is used to label the nuclei Red.
SDS-PAGE Analysis Purified Mitochondria Mouse Monoclonal Antibody (113-1). Confirmation of Integrity and Purity of Antibody.
Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Mitochondria Mouse Monoclonal Antibody (113-1).
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