This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Antibody Type:
Monoclonal Antibody
Application Notes:
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45
SDS-PAGE
Formalin-fixed, paraffin-embedded Rat Heart stained with Muscle Specific Actin Monoclonal Antibody (HHF35 + MSA/953)
Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Muscle Specific Actin Monoclonal Antibody (HHF35 + MSA/953)
SDS-PAGE Analysis Purified Muscle Specific Actin Monoclonal Antibody (HHF35 + MSA/953). Confirmation of Purity and Integrity of Antibody.
Formalin-fixed, paraffin-embedded Rat Lung stained with Muscle Specific Actin MAb (HHF35 + MSA/953)
Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Muscle Specific Actin MAb (HHF35 + MSA/953)
Formalin-fixed, paraffin-embedded Rat Stomach stained with Muscle Specific Actin MAb (HHF35 + MSA/953)
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