Restriction enzymes are a class of endonucleases that recognize specific deoxynucleotide sequences and cleave phosphodiester bonds between two deoxyribonucleotides at specific sites in each strand. Restriction enzymes are important components of the restriction-modification system. The biological role is to protect the host from foreign DNA infection. XbaI, derived from Xanthomonas campestris, is a commonly used type II restriction enzyme. This product is obtained by recombinant protein production technology.
Puffer:
10 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 50% (v/v) Glycerol, 0.05% PS-80 (pH7.4).
Formulierung:
Liquid
Application Verdünnung:
Linearization of in vitro transcription template.Molecular cloning.Genotyping, Southern hybridization.SNPRestriction fragment length polymorphism (RFLP).The optimal working dilution should be determined by the end user.
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