Restriction endonucleases are a class of endonuclease that recognize specific deoxynucleotide sequences and cleave the phosphodiester bond between two deoxyribonucleotides at specific sites in each chain. BspQI derived from Bacillussphaericus, is a commonly used restriction endonuclease. BspQI recognizes the following sequence:5_GGTCTC(N)1_33_CCAGAG(N)5_5.
Puffer:
10 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.5 mg/mL HAS, 50% Glycerol, pH 7.4 at 25C.
Formulierung:
Liquid
Application Verdünnung:
Molecular cloning.GenotypingSouthern hybridizationRestrictive fragment length polymorphism (RFLP).The optimal working dilution should be determined by the end user.
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